Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI00209
Host Cell : CHO-K1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI00209 |
| Description | This cell line is engineered to stably overexpress human sodium voltage-gated channel alpha subunit 10 (SCN10A, Nav1.8) in Chinese hamster ovary (CHO-K1) cells. |
| Product Type | Stable cell line constitutively expressing human ion channel gene(s) |
| Target Gene | SCN10A |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications | in vitro cell-based screening and discovery of ion channel drugs |
| Resistance | Hygromycin |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-Time qPCR assay 2) Manual patch clamp assay 3) Mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | SCN10A |
| Background | The protein encoded by this gene is a tetrodotoxin-resistant voltage-gated sodium channel alpha subunit. The properties of the channel formed by the encoded transmembrane protein can be altered by interaction with different beta subunits. This protein may be involved in the onset of pain associated with peripheral neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
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This SCN10A stable cell line offered a convenient cellular system for our voltage-gated sodium channel studies. The CHO background was suitable for routine handling, and stable target expression helped streamline assay preparation. It is a valuable research tool for Nav1.8 characterization and ion-channel compound assessment.
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