BRCA1 Easy KO Kit (Mouse)

Name: BRCA1 Easy KO Kit (Mouse)
SKU: CC-221
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-221

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Product Information

Gene Information

Cat. No.	CC-221
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Brca1
Gene Name	Brca1 breast cancer 1 [ Mus musculus ]
Species	Mouse
EnsemblID	ENSMUSG00000017146
NCBIGeneID	12189
Uni ProtID	P48754
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	Brca1 breast cancer 1 [ Mus musculus ]
Gene Symbol	BRCA1
GeneID	12189
Uni ProtID	P48754
mRNA Refseq	NM_009764.3
Protein Refseq	NP_033894.3
Chromosome Location	11 D; 11 60.5 cM
Function	DNA binding; RNA binding; damaged DNA binding; enzyme binding; ligase activity; metal ion binding; transcription regulatory region DNA binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding;
Pathway	ATM mediated phosphorylation of repair proteins, organism-specific biosystem; ATM mediated response to DNA double-strand break, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous Recombination Repair, organism-specific biosystem; Homologous recombination repair of replication-independent double-strand breaks, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Recombination, organism-specific biosystem; Meiotic Synapsis, organism-specific biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; PI3K-Akt signaling pathway, conserved biosystem; PluriNetWork, organism-specific biosystem; Recruitment of repair and signaling proteins to double-strand breaks, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem; Ubiquitin mediated proteolysis, conserved biosystem; estrogen signalling, organism-specific biosystem; mRNA processing, organism-specific biosystem;

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