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pComb3Z vector

For research use only. Not intended for any clinical use.
Cat.No.
VPT4041
Description
VPT4041 is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of SfiI cassette for cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and Myc tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest.
Promoter
LacZ
Resistance
Amp
Tag
His6; Myc
Vector Length
3457 bp
Vector Type
Phagemid vector
Vector Map

Background

Applications

Publications

Q & A

Customer Reviews

The pComb3Z vector is the latest development within the pComb vector family. This version provides several significant improvements over its predecessor, pComb3. The pComb3Z vector has not only increased in stability but also includes the introduction of an SfiI cassette. This inclusion allows for the seamless cloning of full Fab, scFv, peptides, and other proteins, thus making it an empowering tool for phage display. pComb3Z contains two tags: 6xHis and Myc tags for purification and detection processes. These tags facilitate the easy isolation of target proteins, yielding pure and characterized products for further analysis or therapeutic use. This further refines and simplifies the cloning process, further increasing its efficiency. An Amber stop codon has been incorporated into the pComb3Z vector that can ‘turn off’ the expression of the pIII fusion protein. This is achieved by switching to a non-suppressor E. Coli strain, which allows for the production of soluble proteins without the requirement for subcloning. This unique feature provides more flexibility and convenience in protein expression studies.
The pComb3Z, a type of phagemid vector, is widely used in the field of molecular biology and genetic engineering due to its unique properties and features. One leading application of the pComb3Z vector is in the area of antibody engineering and production. By using this vector, scientists can produce high-quality recombinant antibodies for diagnostic and therapeutic applications. This ranges from simple detection assays for pathogens and disease markers, to advanced therapeutic treatments such as targeted drug delivery systems and immunotherapy for diseases like cancer and autoimmune disorders. Apart from its use in antibody production, pComb3Z is also used for gene cloning and gene expression studies. It provides an effective platform for researchers to insert, multiply, or express specific genes in bacterial systems, thereby allowing large-scale production of proteins or gene products of interest. This feature is particularly useful for the production of vaccines and other bio-pharmaceuticals. In addition, the pComb3Z vector is used in genetic manipulation studies. Researchers use this vector to modify bacterial genes directly and observe the effects on the subsequent bacterial behavior. The outcome from these experiments provides valuable information necessary for understanding genetic regulation and microbial behavior, which is important in fields as diverse as industrial biotechnology, environmental science, and medical microbiology. Another area where pComb3Z has found wide application is in the field of protein engineering. This vector is used to generate mutant protein libraries, by changing selected amino acid residues. This allows researchers to study the structure-function relationship of proteins at a highly detailed level.
Customer Q&As
What is the Growth Strain of pComb3Z vector?

A: XL1 Blue.

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Customer Reviews
Great!

I've found pComb3Z to be an excellent choice for phage display applications. Its design allows for easy insertion of recombinant DNA and effective propagation in bacteria.

Canada

02/05/2023

A smooth experience

Working with pComb3Z vector has been a smooth experience. It has high cloning efficiency and works great for displaying peptides or antibodies on phage surfaces.

United Kingdom

08/25/2020

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