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pComb3X vector

Cat.No.
VPT4012
Product description
VPT4012 is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of SfiI cassette for cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest.
Vector type
Phagemid vector
Vector length
3451 bp
Tag
His6;HA
Promotor
LacZ
Resistance
Amp
pComb3X vector

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