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Cat.No. : CSC-RO0895 Host Cell: HEK293T
| Cat. No. | CSC-RO0895 |
| Description | This cell line is engineered to stably overexpress monkey PVRIG. |
| Gene | PVRIG |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Monkey PVRIG Stable Cell Line - HEK293T is a cell line derived from human embryonic kidney cells (HEK293T) that has been genetically modified to stably express Monkey PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain) protein. PVRIG is an immune regulatory protein associated with T-cell activation and immune responses. The uniqueness of this cell line lies in its utility for studying the function of Monkey PVRIG protein, its signaling pathways, and interactions with other immune molecules.
A: HEK293T cells are commonly used due to their high scalability and ease of manipulation, making them suitable for gene expression and protein production research. Choosing HEK293T cells for stable expression of Monkey PVRIG is based on their widespread availability, efficient protein expression capabilities, and suitability for biological research.
A: The culture conditions for Monkey PVRIG Stable Cell Line - HEK293T typically involve the use of high-glucose culture media (such as DMEM) supplemented with appropriate concentrations of antibiotics like penicillin/streptomycin to maintain cell stability. The culture temperature is usually set at 37°C, with a CO2 concentration of 5%, and regular medium changes to support cell growth.
A: Monkey PVRIG expression levels in Monkey PVRIG Stable Cell Line - HEK293T can be determined through various methods, including Western blotting, flow cytometry analysis, and quantitative PCR, among others. These methods can be used to detect the expression levels of Monkey PVRIG protein and quantify it within the cells.
A: Monkey PVRIG and human PVRIG exhibit differences in their amino acid sequences, which may lead to functional distinctions. Monkey PVRIG Stable Cell Line - HEK293T can be used to investigate the function and signaling mechanisms of Monkey PVRIG protein in monkey cells, helping us understand the differences between Monkey PVRIG and human PVRIG and their potential impact on the immune system.
A: Monkey PVRIG Stable Cell Line - HEK293T can be employed to study the role of Monkey PVRIG protein in T-cell activation and immune regulation. Co-culturing this cell line with other cells or immune molecules allows the assessment of Monkey PVRIG's involvement in immune responses, thereby facilitating in-depth exploration of its immune regulatory mechanisms.
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