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Cat.No. : CSC-RO0856 Host Cell: CHO-K1
| Cat. No. | CSC-RO0856 |
| Description | This cell line is engineered to stably overexpress monkey NT5E (also named as CD73). |
| Gene | NT5E |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Monkey NT5E Stable Cell Line - CHO-K1 is typically constructed by stably introducing the monkey-derived NT5E gene into CHO-K1 cells. This can be achieved through techniques such as transfection or viral transduction.
A: This cell line can be used in biomedical research to study the function of NT5E (CD73), immunology research, drug screening, and research related to diseases such as cancer and autoimmune disorders.
A: Common techniques and methods when using Monkey NT5E Stable Cell Line - CHO-K1 for experiments include cell culture, flow cytometry, enzyme-linked immunosorbent assay (ELISA), among others, to investigate NT5E activity and related biological processes.
A: Evaluating the drug effect on NT5E typically involves testing at different drug concentrations to understand its impact on cell signaling and immune responses.
A: This cell line can simulate the biological processes of related autoimmune diseases or cancer by introducing relevant genes or cytokines and applying appropriate stimuli to study their mechanisms and treatment strategies.
A: This cell line can be used to evaluate the effects of potential drugs on NT5E, providing an important testing platform, especially in the field of cancer therapy.
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