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Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
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Powerful Tn5 Transposase for DNA insertion and random library construction.
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Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
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High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
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Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
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Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Cat.No. : CSC-RO1020 Host Cell: HEK293T
| Cat. No. | CSC-RO1020 |
| Description | HEK293-LAG3 cell line is engineered to stably overexpress monkey LAG3. |
| Gene | LAG3 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Try gradually adapting to low serum conditions or add specific growth factors and supplements to the culture medium to promote cell growth.
A: Optimize protein lysis conditions and antibody selection to enhance protein extraction and immunoprecipitation efficiency, and optimize washing steps to reduce non-specific binding.
A: Cell morphology changes could be a toxic response to the drug. Adjust the drug concentration or use different drug treatment durations to assess its impact on cells.
A: Optimize transfection conditions and components of the CRISPR/Cas9 system, such as sgRNA design and Cas9 protein concentration, to ensure efficient gene editing while minimizing off-target effects.
A: Check and optimize culture conditions, such as medium, temperature, and CO₂ concentration, reduce physical or chemical stress, and consider using cell protectants.
A: Optimize the staining protocol for flow cytometry, such as antibody concentration and incubation time, to ensure cell viability and reduce the impact of cell death on analysis results.
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