Monkey LAG3 Stable Cell Line-HEK293T

Name: Monkey LAG3 Stable Cell Line-HEK293T
SKU: CSC-RO1020
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1020

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO1020
Description	HEK293-LAG3 cell line is engineered to stably overexpress monkey LAG3.
Target Gene	LAG3
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How to improve the growth of Monkey LAG3 Stable Cell Line-HEK293 under low serum conditions?

A: Try gradually adapting to low serum conditions or add specific growth factors and supplements to the culture medium to promote cell growth.

How to enhance the detection efficiency of LAG3 protein in immunoprecipitation experiments with Monkey LAG3 Stable Cell Line-HEK293?

A: Optimize protein lysis conditions and antibody selection to enhance protein extraction and immunoprecipitation efficiency, and optimize washing steps to reduce non-specific binding.

What causes cell morphology changes in Monkey LAG3 Stable Cell Line-HEK293 during drug screening experiments?

A: Cell morphology changes could be a toxic response to the drug. Adjust the drug concentration or use different drug treatment durations to assess its impact on cells.

How to effectively perform CRISPR/Cas9 gene editing in Monkey LAG3 Stable Cell Line-HEK293 cells?

A: Optimize transfection conditions and components of the CRISPR/Cas9 system, such as sgRNA design and Cas9 protein concentration, to ensure efficient gene editing while minimizing off-target effects.

How to adjust when there is an abnormally high rate of apoptosis in Monkey LAG3 Stable Cell Line-HEK293 under certain experimental conditions?

A: Check and optimize culture conditions, such as medium, temperature, and CO₂ concentration, reduce physical or chemical stress, and consider using cell protectants.

How to improve the stability of LAG3 surface marker detection in flow cytometry analysis of Monkey LAG3 Stable Cell Line-HEK293?

A: Optimize the staining protocol for flow cytometry, such as antibody concentration and incubation time, to ensure cell viability and reduce the impact of cell death on analysis results.

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