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Cat.No. : CSC-RO0560 Host Cell: HEK293T
| Cat. No. | CSC-RO0560 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Cyno GPC3. |
| Gene | GPC3 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: By using Monkey GPC3 Stable Cell Line - HEK293T, the role of GPC3 in cell signaling can be studied through various biochemical and molecular biology methods. For instance, identifying proteins that interact with GPC3 using immunoprecipitation and mass spectrometry, and monitoring GPC3-regulated signaling pathways using reporter gene systems.
A: For antibody screening, optimizing the culture conditions of Monkey GPC3 Stable Cell Line - HEK293T to ensure stable expression of GPC3 is essential. Additionally, employing highly sensitive detection methods, such as flow cytometry or ELISA, is crucial to efficiently screen and identify antibodies with high specificity and affinity for GPC3.
A: Monkey GPC3 Stable Cell Line - HEK293T has broad prospects in tumor immunotherapy research. As GPC3 is a potential target in various tumors, this cell line can be used to study the role of GPC3 in the tumor microenvironment and evaluate immunotherapeutic strategies targeting GPC3, such as CAR-T cell therapy.
A: When conducting genome editing experiments, selecting appropriate targets and editing tools, like the CRISPR/Cas9 system, is important. Considering the genetic background of HEK293T cells, appropriate screening and validation steps are needed to ensure the specificity and efficiency of gene editing and to avoid off-target editing events.
A: Monkey GPC3 Stable Cell Line - HEK293T can be used to study the potential role of GPC3 in liver fibrosis and cirrhosis. Since GPC3 is closely related to the development of liver diseases, this cell line provides a valuable platform for exploring the role of GPC3 in liver pathologies and its potential as a therapeutic target.
A: Monkey GPC3 Stable Cell Line - HEK293T, with its stable expression of monkey glypican-3 (GPC3), holds significant potential in liver cancer research. GPC3 is closely associated with the development and progression of liver cancer, and this cell line can be used to study the role of GPC3 in liver cancer cell proliferation, invasion, and metastasis, as well as to assess therapeutic strategies targeting GPC3.
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