Monkey GPC3 Stable Cell Line - HEK293T

Name: Monkey GPC3 Stable Cell Line - HEK293T
SKU: CSC-RO0560
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0560

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0560
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Cyno GPC3.
Target Gene	GPC3
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How can the role of GPC3 in cell signaling be studied using Monkey GPC3 Stable Cell Line - HEK293T?

A: By using Monkey GPC3 Stable Cell Line - HEK293T, the role of GPC3 in cell signaling can be studied through various biochemical and molecular biology methods. For instance, identifying proteins that interact with GPC3 using immunoprecipitation and mass spectrometry, and monitoring GPC3-regulated signaling pathways using reporter gene systems.

How should the experimental design be optimized for antibody screening using Monkey GPC3 Stable Cell Line - HEK293T?

A: For antibody screening, optimizing the culture conditions of Monkey GPC3 Stable Cell Line - HEK293T to ensure stable expression of GPC3 is essential. Additionally, employing highly sensitive detection methods, such as flow cytometry or ELISA, is crucial to efficiently screen and identify antibodies with high specificity and affinity for GPC3.

What are the prospects of Monkey GPC3 Stable Cell Line - HEK293T in tumor immunotherapy research?

A: Monkey GPC3 Stable Cell Line - HEK293T has broad prospects in tumor immunotherapy research. As GPC3 is a potential target in various tumors, this cell line can be used to study the role of GPC3 in the tumor microenvironment and evaluate immunotherapeutic strategies targeting GPC3, such as CAR-T cell therapy.

What key factors should be considered when conducting genome editing experiments with Monkey GPC3 Stable Cell Line - HEK293T?

A: When conducting genome editing experiments, selecting appropriate targets and editing tools, like the CRISPR/Cas9 system, is important. Considering the genetic background of HEK293T cells, appropriate screening and validation steps are needed to ensure the specificity and efficiency of gene editing and to avoid off-target editing events.

What is the potential role of Monkey GPC3 Stable Cell Line - HEK293T in studying liver fibrosis and cirrhosis?

A: Monkey GPC3 Stable Cell Line - HEK293T can be used to study the potential role of GPC3 in liver fibrosis and cirrhosis. Since GPC3 is closely related to the development of liver diseases, this cell line provides a valuable platform for exploring the role of GPC3 in liver pathologies and its potential as a therapeutic target.

What is the potential application of Monkey GPC3 Stable Cell Line - HEK293T in liver cancer research?

A: Monkey GPC3 Stable Cell Line - HEK293T, with its stable expression of monkey glypican-3 (GPC3), holds significant potential in liver cancer research. GPC3 is closely associated with the development and progression of liver cancer, and this cell line can be used to study the role of GPC3 in liver cancer cell proliferation, invasion, and metastasis, as well as to assess therapeutic strategies targeting GPC3.

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