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Cat.No. : CSC-RO0536 Host Cell: HEK293T
| Cat. No. | CSC-RO0536 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Cyno CD40. |
| Gene | CD40 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey CD40 Stable Cell Line - HEK293T cell line is constructed by stably expressing monkey-derived CD40 molecules in human embryonic kidney cells HEK293T through genetic engineering techniques. The unique value of this cell line lies in its ability to simulate the interactions between monkey-derived CD40 molecules in immune cell interactions, which is crucial for studying the immune regulatory role of the CD40-CD40L (CD154) signaling pathway in non-human primates. Moreover, due to the high genetic and physiological similarity between monkeys and humans, this cell line can serve as a model for studying the human immune system, especially when developing therapeutic strategies for human diseases.
A: In vaccine development, the Monkey CD40 Stable Cell Line - HEK293T cell line can be used to assess the impact of vaccine candidates on the activation of immune cells. By monitoring the activation status of the CD40 signaling pathway, researchers can predict whether the vaccine can induce an effective immune response. In addition, this cell line can also be used to screen for immunomodulators that can enhance the efficacy of vaccines, thereby optimizing vaccine formulations.
A: In the study of autoimmune diseases, the Monkey CD40 Stable Cell Line - HEK293T cell line can be used to simulate the process of autoantigen presentation. By expressing specific autoantigens in this cell line and observing their activating effects on immune cells, scientists can better understand the initiation mechanism of autoimmune responses and explore potential therapeutic targets.
A: In the drug screening process, the Monkey CD40 Stable Cell Line - HEK293T cell line can serve as a high-throughput screening platform for identifying drug molecules that can modulate the CD40 signaling pathway. These drugs may have the potential to suppress or enhance immune responses, which is significant for developing new drugs to treat immune-related diseases.
A: To enhance the applicability of the Monkey CD40 Stable Cell Line - HEK293T cell line in research, scientists can further optimize the stability and expression levels of the cell line through genetic editing techniques. Additionally, improving culture conditions and screening strategies can enhance the consistency and reproducibility of the cell line, ensuring its reliability under different experimental conditions.
A: In studying the development of immune cells, the Monkey CD40 Stable Cell Line - HEK293T cell line can serve as a tool to investigate the role of the CD40 signaling pathway in the maturation and functional differentiation of immune cells. By expressing CD40 at different stages of cell development and observing its effects on cell phenotype and function, the key role of CD40 in immune cell development can be revealed.
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