Cat.No. : CSC-RO0874 Host Cell: HEK293T
| Cat. No. | CSC-RO0874 |
| Description | This cell line is engineered to stably overexpress human PVRIG. |
| Gene | PVRIG |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The PVRIG gene encodes for the protein V-set domain-containing T cell immunoglobulin and ITIM domain-containing protein (VIR), also known as PVRIG. This protein is a recently discovered inhibitory receptor expressed on the surface of T cells and natural killer (NK) cells. PVRIG plays a role in regulating immune responses by inhibiting the activation and function of these immune cells. It does so by interacting with other proteins on the surface of antigen-presenting cells and T cells.
A: The PVRIG gene contributes to immune response regulation by inhibiting excessive immune activation and preventing autoimmunity. By interacting with the CD81 and CD82 receptors on T cells and NK cells, PVRIG can inhibit their responses to certain antigens. This inhibition helps maintain immune tolerance and prevents the immune system from attacking healthy cells.
A: Mutations in the PVRIG gene are not commonly associated with specific diseases. However, dysregulation of PVRIG expression or function may lead to alterations in immune responses, potentially affecting the body's ability to mount effective defenses against pathogens or cancer cells.
A: The expression of the PVRIG gene is regulated by various factors, including cytokines and transcription factors. The activation of T cells and NK cells can lead to the upregulation of PVRIG expression. Additionally, regulatory mechanisms, such as epigenetic modifications and post-translational modifications, can influence the expression of PVRIG, ensuring proper immune responses.
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The Human PVRIG Stable Cell Line - HEK293T represents a novel and innovative tool for researchers studying immune regulation and cancer immunotherapy. Its development provides new opportunities to investigate the role of PVRIG in immune responses and its potential as a therapeutic target.
The provider of the Human PVRIG Stable Cell Line - HEK293T is committed to continuous updates and improvements, ensuring that the cell line remains relevant and effective as the field evolves. Researchers can benefit from the latest advancements in cell line technology and applications.
The Human PVRIG Stable Cell Line - HEK293T is developed using a state-of-the-art platform, which incorporates cutting-edge gene editing techniques and cell culture methods. This ensures the production of high-quality and genetically stable cell lines that can be used with confidence in various research applications.
The provider offers a range of editing strategies for the Human PVRIG Stable Cell Line - HEK293T, allowing researchers to choose the most suitable approach for their specific experimental needs. This flexibility enables customization and optimization of the cell line to best suit individual research goals and objectives.
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