Human NCR3 Stable Cell Line - CHO-K1

Name: Human NCR3 Stable Cell Line - CHO-K1
SKU: CSC-RO0257
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0257

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0257
Description	Human NCR3 Stable Cell Line - CHO-K1 is engineered to stably overexpress human natural cytotoxicity triggering receptor 3 (NCR3).
Target Gene	NCR3
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens natural cytotoxicity triggering receptor 3 DNA.
Gene Symbol	NCR3
Synonyms	1C7, MALS, CD337, LY117, NKp30, NCR3
m RNA Refseq	NM_147130.1

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the origin of the Human NCR3 Stable Cell Line - CHO-K1?

A: The Human NCR3 Stable Cell Line - CHO-K1 is derived from Chinese Hamster Ovary (CHO-K1) cells, which are a common host cell line used in biotechnology and research.

Are there any unique considerations or challenges associated with using the Human NCR3 Stable Cell Line - CHO-K1 in research compared to other cell lines?

A: Considerations may include optimizing culture conditions for recombinant protein expression, studying the function of NCR3 in the context of CHO-K1 cells, and exploring interactions with other cellular components. Researchers should be aware of the specific properties of CHO-K1 cells for their experiments.

What are the potential limitations or caveats when using the Human NCR3 Stable Cell Line - CHO-K1 in research, and how can they be addressed?

A: Limitations may include potential differences in NCR3 expression compared to native cells, variations in receptor function due to the cellular context, and the need for appropriate controls in experiments. Researchers can address these issues by carefully designing their experiments, using validated reagents, and performing functional assays.

What are the recommended storage and revival procedures for the Human NCR3 Stable Cell Line - CHO-K1?

A: The storage and revival procedures for the Human NCR3 Stable Cell Line - CHO-K1 may involve cryopreservation in a suitable medium at a specific temperature and subsequent thawing and culturing as needed. Detailed protocols should be provided by the cell line provider or developed based on best practices for CHO-K1 cells.

What are the primary applications of the Human NCR3 Stable Cell Line - CHO-K1 in research or biotechnology?

A: The Human NCR3 Stable Cell Line - CHO-K1 can be used for various applications, including studying the NCR3 receptor's function in natural killer (NK) cell biology, investigating immune responses, and exploring the role of NCR3 in cellular interactions and cytotoxicity.

What is the specific genotype and phenotype of the Human NCR3 Stable Cell Line - CHO-K1?

A: The specific genotype and phenotype of the Human NCR3 Stable Cell Line - CHO-K1 would depend on the genetic modifications introduced to express the NCR3 (Natural Cytotoxicity Triggering Receptor 3) gene. CHO-K1 cells are known for their ability to grow in suspension culture and are often used for recombinant protein expression.

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