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Cat.No. : CSC-RO0257 Host Cell: CHO-K1
| Cat. No. | CSC-RO0257 |
| Description | Human NCR3 Stable Cell Line - CHO-K1 is engineered to stably overexpress human natural cytotoxicity triggering receptor 3 (NCR3). |
| Gene | NCR3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human NCR3 Stable Cell Line - CHO-K1 is derived from Chinese Hamster Ovary (CHO-K1) cells, which are a common host cell line used in biotechnology and research.
A: Considerations may include optimizing culture conditions for recombinant protein expression, studying the function of NCR3 in the context of CHO-K1 cells, and exploring interactions with other cellular components. Researchers should be aware of the specific properties of CHO-K1 cells for their experiments.
A: Limitations may include potential differences in NCR3 expression compared to native cells, variations in receptor function due to the cellular context, and the need for appropriate controls in experiments. Researchers can address these issues by carefully designing their experiments, using validated reagents, and performing functional assays.
A: The storage and revival procedures for the Human NCR3 Stable Cell Line - CHO-K1 may involve cryopreservation in a suitable medium at a specific temperature and subsequent thawing and culturing as needed. Detailed protocols should be provided by the cell line provider or developed based on best practices for CHO-K1 cells.
A: The Human NCR3 Stable Cell Line - CHO-K1 can be used for various applications, including studying the NCR3 receptor's function in natural killer (NK) cell biology, investigating immune responses, and exploring the role of NCR3 in cellular interactions and cytotoxicity.
A: The specific genotype and phenotype of the Human NCR3 Stable Cell Line - CHO-K1 would depend on the genetic modifications introduced to express the NCR3 (Natural Cytotoxicity Triggering Receptor 3) gene. CHO-K1 cells are known for their ability to grow in suspension culture and are often used for recombinant protein expression.
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