Human KLRD1/KLRC2 Stable Cell Line - HEK293T

Name: Human KLRD1/KLRC2 Stable Cell Line - HEK293T
SKU: CSC-RO0903
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0903

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0903
Description	This cell line is engineered to stably overexpress human KLRD1/KLRC2 (also named as CD94-NKG2C).
Target Gene	KLRD1/KLRC2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Customer Reviews

Customer Q&As

What are the unique applications of the Human KLRD1/KLRC2 Stable Cell Line - HEK293T in studying Natural Killer (NK) cell-mediated immune responses?

A: The Human KLRD1/KLRC2 Stable Cell Line - HEK293T, created by stably transfecting KLRD1 and KLRC2 genes into HEK293T cells, offers uniqueness in studying NK cell-mediated immune responses. It efficiently expresses two key receptors regulating NK cell activity, CD94 (encoded by KLRD1) and NKG2 (encoded by KLRC2). This allows researchers to precisely evaluate the impact of these receptors on NK cell activity and their mechanisms in various pathological conditions in an in vitro setting.

What unique characteristics does the Human KLRD1/KLRC2 Stable Cell Line - HEK293T exhibit in immunomodulatory studies?

A: : The unique aspect of the Human KLRD1/KLRC2 Stable Cell Line - HEK293T in immunomodulatory studies is its ability to mimic certain immune functions of natural killer (NK) cells through the expression of KLRD1 and KLRC2 genes. This allows researchers to study the activity of NK cell receptors and how they influence immune responses in an in vitro setting. Additionally, the high transfection efficiency and stable gene expression of the HEK293T cells further enhance their utility in immunological research.

What impact does the co-expression of KLRD1 and KLRC2 have on NK cell-mediated cytotoxicity in the Human KLRD1/KLRC2 Stable Cell Line - HEK293T?

A: In the Human KLRD1/KLRC2 Stable Cell Line - HEK293T, the co-expression of KLRD1 and KLRC2 simulates the critical role of NK cells in immune responses. These receptors play significant roles in NK cell recognition and elimination of infected or transformed cells. By studying their co-expression, scientists can better understand how NK cells regulate their cytotoxicity and how these receptors interact to modulate immune responses.

What are the advantages and limitations of using the Human KLRD1/KLRC2 Stable Cell Line - HEK293T for drug screening?

A: The advantage of using the Human KLRD1/KLRC2 Stable Cell Line - HEK293T for drug screening lies in its ability to assess the impact of drugs on NK cell receptor activity, especially in anti-tumor and antiviral therapies. The high transfection efficiency and stable gene expression of this cell line also facilitate rapid high-throughput screening. However, its limitation is that, as an in vitro model, it cannot fully replicate the complex immune environment in the human body, particularly in scenarios involving multiple types of immune cells and signaling pathways.

What is the potential application of the Human KLRD1/KLRC2 Stable Cell Line - HEK293T in simulating viral infection processes?

A: The Human KLRD1/KLRC2 Stable Cell Line - HEK293T holds significant potential in simulating viral infection processes. By expressing specific NK cell receptors, this cell line can be used to study how NK cells recognize and respond to viral infections. This is particularly important for understanding mechanisms of viral immune evasion and developing new antiviral strategies.

How can effective gene editing be performed in the Human KLRD1/KLRC2 Stable Cell Line - HEK293T to study the functions of KLRD1 and KLRC2?

A: In the Human KLRD1/KLRC2 Stable Cell Line - HEK293T, gene editing to study the functions of KLRD1 and KLRC2 can be achieved using the CRISPR/Cas9 technology. By designing specific guide RNAs (gRNAs), it is possible to precisely target and modify these genes, thereby observing the impact of gene knockout or knock-in on the function of NK cell receptors. This is crucial for a deeper understanding of the role these receptors play in immune regulation.

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