Human KIT Stable Cell Line-CHO-K1(GS-/-)

Name: Human KIT Stable Cell Line-CHO-K1(GS-/-)
SKU: CSC-RO1071
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1071

Host Cell : CHO-K1(GS-/-) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1071
Description	CHO-K1(GS-/-)-KIT cell line is engineered to stably overexpress human KIT.
Target Gene	KIT
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1(GS-/-)
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	Drug screening and biological assays
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
Gene Symbol	KIT
Synonyms	PBT; SCFR; C-Kit; CD117
Gene Description	v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
GeneID	3815
Uni ProtID	P10721
mRNA Refseq	NM_000222.2
Protein Refseq	NP_000213.1
Chromosome Location	4q11-q12
Function	ATP binding; cytokine binding; metal ion binding; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity;
Pathway	Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
MIM	164920

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