Human KIT(Fc) HEK293 Cell Lysate

Name: Human KIT(Fc) HEK293 Cell Lysate
SKU: CLOE-0695
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0695 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0695
Description	This cell lysate over-expresses human KIT (P10721-2) (Met1-Thr516) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses human KIT (P10721-2) (Met1-Thr516) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	KIT
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
Gene Symbol	KIT
Synonyms	PBT; SCFR; C-Kit; CD117
Gene Description	v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
GeneID	3815
Uni ProtID	P10721
mRNA Refseq	NM_000222.2
Protein Refseq	NP_000213.1
Chromosome Location	4q11-q12
Function	ATP binding; cytokine binding; metal ion binding; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity;
Pathway	Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
MIM	164920

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