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Cat.No. : CSC-RO0908 Host Cell: B16F10
| Cat. No. | CSC-RO0908 |
| Description | This cell line is engineered to stably overexpress human CTAG1B (also named as NY-ESO-1). |
| Gene | CTAG1B |
| Gene Species | Homo sapiens (Human) |
| Host Cell | B16F10 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CTAG1B Stable Cell Line - B16F10 is a stable cell line derived from the mouse melanoma cell line B16F10. This cell line has been stably transfected or engineered to express the human CTAG1B gene, which is typically highly expressed in various cancers, making it suitable for cancer research and drug screening.
A: B16F10 is a commonly used mouse melanoma cell line known for its ease of cultivation, rapid proliferation, and high invasive and metastatic capabilities. It was chosen as the background because it allows researchers to simulate cancer dissemination and metastasis processes while observing the role of the CTAG1B gene in these processes.
A: The CTAG1B gene is typically stably expressed in B16F10 cells, meaning its expression is not significantly affected by external stimuli or environmental changes. This allows researchers to control the expression of the CTAG1B gene to some extent for relevant experiments and studies.
A: The Human CTAG1B Stable Cell Line - B16F10 can be used in multiple research fields, including cancer biology, immunology, and drug development. It can be used to study the role of the CTAG1B gene in cancer development, spread, and therapy, as well as for developing drugs or immunotherapies targeting CTAG1B.
A: In addition to the Human CTAG1B Stable Cell Line - B16F10, there are other cell lines and models used in cancer research. These include different types of cancer cell lines and mouse models that can be used for various experiments and studies.
A: The CTAG1B gene is significant in tumor biology because it is typically highly expressed in various cancers, including breast cancer, lung cancer, and malignant melanoma. It is considered a tumor-associated antigen (TAA) and may be relevant to the development of immunotherapies and cancer vaccines.
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