Human CTAG1B Stable Cell Line - B16F10

Name: Human CTAG1B Stable Cell Line - B16F10
SKU: CSC-RO0908
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0908

Host Cell : B16F10 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0908
Description	This cell line is engineered to stably overexpress human CTAG1B (also named as NY-ESO-1).
Target Gene	CTAG1B
Gene Species	Homo sapiens (Human)
Host Cell	B16F10
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CTAG1B cancer/testis antigen 1B [ Homo sapiens ]
Gene Symbol	CTAG1B
Synonyms	CTAG; ESO1; CT6.1; CTAG1; LAGE-2; LAGE2B; NY-ESO-1
GeneID	1485
Uni ProtID	P78358
mRNA Refseq	NM_001327.2
Protein Refseq	NP_001318.1
Chromosome Location	Xq28
MIM	300156

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What type of cell line is the Human CTAG1B Stable Cell Line - B16F10, and what are its origins and characteristics?

A: The Human CTAG1B Stable Cell Line - B16F10 is a stable cell line derived from the mouse melanoma cell line B16F10. This cell line has been stably transfected or engineered to express the human CTAG1B gene, which is typically highly expressed in various cancers, making it suitable for cancer research and drug screening.

Why was B16F10 chosen as the background for this cell line?

A: B16F10 is a commonly used mouse melanoma cell line known for its ease of cultivation, rapid proliferation, and high invasive and metastatic capabilities. It was chosen as the background because it allows researchers to simulate cancer dissemination and metastasis processes while observing the role of the CTAG1B gene in these processes.

What is the expression pattern of the CTAG1B gene in B16F10 cells?

A: The CTAG1B gene is typically stably expressed in B16F10 cells, meaning its expression is not significantly affected by external stimuli or environmental changes. This allows researchers to control the expression of the CTAG1B gene to some extent for relevant experiments and studies.

In which research fields and applications can the Human CTAG1B Stable Cell Line - B16F10 be used?

A: The Human CTAG1B Stable Cell Line - B16F10 can be used in multiple research fields, including cancer biology, immunology, and drug development. It can be used to study the role of the CTAG1B gene in cancer development, spread, and therapy, as well as for developing drugs or immunotherapies targeting CTAG1B.

Are there other cell lines or models related to the Human CTAG1B Stable Cell Line - B16F10?

A: In addition to the Human CTAG1B Stable Cell Line - B16F10, there are other cell lines and models used in cancer research. These include different types of cancer cell lines and mouse models that can be used for various experiments and studies.

What is the significance of the CTAG1B gene in tumor biology?

A: The CTAG1B gene is significant in tumor biology because it is typically highly expressed in various cancers, including breast cancer, lung cancer, and malignant melanoma. It is considered a tumor-associated antigen (TAA) and may be relevant to the development of immunotherapies and cancer vaccines.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *