Cat.No. : CSC-RO0898 Host Cell: HCT15
| Cat. No. | CSC-RO0898 |
| Description | This cell line is engineered to stably overexpress human CD274-TACSTD2 (also named as PDL1-TROP2). |
| Gene | CD274-TACSTD2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HCT15 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line, by stably expressing CD274 (PD-L1) and TACSTD2 (EpCAM) - two tumor-associated antigens, can be used to evaluate the efficacy of immune checkpoint inhibitors targeting these antigens. Particularly, it serves as a valuable model in studying the role of the PD-1/PD-L1 pathway in tumor immune evasion.
A: Specificity can be evaluated by comparing the response of this cell line to specific drugs with the response of other cell lines that do not express or express low levels of CD274 and TACSTD2. Additionally, employing gene knockout or knockdown techniques targeting these proteins and observing changes in drug sensitivity is another method to assess specificity.
A: As this cell line expresses two proteins closely associated with tumor progression and immune evasion, it better simulates the complex interactions in the tumor microenvironment. This is particularly important for studying tumor immunology and developing new immunotherapeutic strategies.
A: This cell line can be used to study how tumor cells affect immune cells, especially the activation and function of T cells, through the expression of PD-L1 and EpCAM. This is crucial for understanding how tumors resist attacks from the immune system and for providing potential targets for immunotherapy.
A: By analyzing the response of this cell line to common chemotherapeutic agents, the relationship between the expression levels of CD274 and TACSTD2 and tumor drug resistance can be studied. Additionally, the role of these proteins in tumor cells evading immune checkpoint inhibitor treatment can be explored.
A: Key factors include assessing how the expression of CD274 and TACSTD2 correlates with the aggressiveness, metastatic potential, and prognosis of tumors. Moreover, variations in these proteins and their expression patterns in different types and stages of cancer should also be studied.
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