Human CD274-TACSTD2 Stable Cell Line - HCT15

Name: Human CD274-TACSTD2 Stable Cell Line - HCT15
SKU: CSC-RO0898
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0898

Host Cell : HCT15 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0898
Description	This cell line is engineered to stably overexpress human CD274-TACSTD2 (also named as PDL1-TROP2).
Target Gene	CD274-TACSTD2
Gene Species	Homo sapiens (Human)
Host Cell	HCT15
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What are the potential applications of the Human CD274-TACSTD2 Stable Cell Line - HCT15 in tumor immunotherapy research?

A: This cell line, by stably expressing CD274 (PD-L1) and TACSTD2 (EpCAM) - two tumor-associated antigens, can be used to evaluate the efficacy of immune checkpoint inhibitors targeting these antigens. Particularly, it serves as a valuable model in studying the role of the PD-1/PD-L1 pathway in tumor immune evasion.

How should the specificity of drug response be assessed when using the Human CD274-TACSTD2 Stable Cell Line - HCT15 for drug screening?

A: Specificity can be evaluated by comparing the response of this cell line to specific drugs with the response of other cell lines that do not express or express low levels of CD274 and TACSTD2. Additionally, employing gene knockout or knockdown techniques targeting these proteins and observing changes in drug sensitivity is another method to assess specificity.

What are the advantages of the Human CD274-TACSTD2 Stable Cell Line - HCT15 in simulating the tumor microenvironment?

A: As this cell line expresses two proteins closely associated with tumor progression and immune evasion, it better simulates the complex interactions in the tumor microenvironment. This is particularly important for studying tumor immunology and developing new immunotherapeutic strategies.

What role does the Human CD274-TACSTD2 Stable Cell Line - HCT15 play in studying the interactions between tumor cells and immune cells?

A: This cell line can be used to study how tumor cells affect immune cells, especially the activation and function of T cells, through the expression of PD-L1 and EpCAM. This is crucial for understanding how tumors resist attacks from the immune system and for providing potential targets for immunotherapy.

How is the Human CD274-TACSTD2 Stable Cell Line - HCT15 applied in studying tumor drug resistance?

A: By analyzing the response of this cell line to common chemotherapeutic agents, the relationship between the expression levels of CD274 and TACSTD2 and tumor drug resistance can be studied. Additionally, the role of these proteins in tumor cells evading immune checkpoint inhibitor treatment can be explored.

What are the key factors to consider when using the Human CD274-TACSTD2 Stable Cell Line - HCT15 in cancer biomarker research?

A: Key factors include assessing how the expression of CD274 and TACSTD2 correlates with the aggressiveness, metastatic potential, and prognosis of tumors. Moreover, variations in these proteins and their expression patterns in different types and stages of cancer should also be studied.

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