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Cat.No. : CSC-RO0907 Host Cell: MDCK-II
| Cat. No. | CSC-RO0907 |
| Description | This cell line is engineered to stably overexpress human B2M/FCGRT. |
| Gene | B2M/FCGRT |
| Gene Species | Homo sapiens (Human) |
| Host Cell | MDCK-II |
| Host Cell Species | Canis lupus familiaris (Dog) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human B2M/FCGRT Stable Cell Line - MDCKⅡ, due to its stable expression of human Beta-2 microglobulin (B2M) and Fc gamma receptor transporters (FCGRT), can be used to study the transport characteristics of drugs across cell layers, especially for the transmembrane transport of Immunoglobulin G (IgG) and related biologics.
A: This can be assessed by adding fluorescently labeled IgG to the culture medium and observing and quantifying the accumulation of fluorescent material inside the Human B2M/FCGRT Stable Cell Line - MDCKⅡ cells.
A: This can be done by adding liver microsomes or specific enzyme inducers to the culture medium to simulate the action of hepatic microsomal enzymes in the metabolism of drugs in Human B2M/FCGRT Stable Cell Line - MDCKⅡ.
A: This can be evaluated through cell viability assays, such as MTT or WST-1 assays, to assess the response and sensitivity of Human B2M/FCGRT Stable Cell Line - MDCKⅡ to different concentrations of cancer drugs.
A: Advanced gene-editing tools like CRISPR/Cas9 or TALENs can be used, and optimizing the design of target sequences and delivery methods can enhance the efficiency of gene knock-in or knock-out. Also, choosing appropriate selection strategies and clonal isolation techniques is crucial.
A: This cell line, by expressing B2M and FCGRT, can simulate key functions of the human immune system, especially in studying Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) responses.
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