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human MDM2 (NM_002392) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV18002L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No. LV18002L
Description Lentiviral particles of Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2.
Product Type Premade Lentivirus Particles
Gene MDM2
Species Human
Insert Size 1494 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name MDM2 Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) [ Homo sapiens ]
Gene Symbol MDM2
Synonyms HDMX; hdm2; ACTFS
GeneID 4193
Uni ProtID Q00987
mRNA Refseq NM_002392.5
Protein Refseq NP_002383.2
Chromosome Location 12q14.3-q15
Function enzyme binding; identical protein binding; p53 binding; protein binding; scaffold protein binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity; zinc ion binding;
Pathway AKT phosphorylates targets in the cytosol, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem;
MIM 164785
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  1. Liu Y, Chen P, Qi D, et al. Glaucocalyxin A inhibits the malignancies of gastric cancer cells by downregulating MDM2 and RNF6 via MiR-3658 and the SMG1-UPF mRNA decay pathway[J]. Frontiers in Oncology, 2022, 12: 871169.
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