EF1α-Null-Puro Lentivirus

Lentiviral vectors (LVs) are viral vector systems modified from human immunodeficiency virus (HIV-1 virus). They can efficiently introduce target genes (or RNAi) into primary cells or cell lines of animals and humans, and have the ability to infect both dividing and non-dividing cells.

The lentiviral vector genome is a single-stranded positive-strand RNA. After entering the cell, the genome is reversed into DNA by the reverse transcriptase carried by itself in the cytoplasm to form a DNA pre-integration complex. After entering the cell nucleus, the DNA is integrated into the cell genome. The integrated DNA is transcribed into mRNA, returned to the cytoplasm, and expressed the target gene or fragment.

The gene expression or RNAi interference mediated by the lentiviral vector is continuous and stable, and its target gene is integrated into the host cell genome and divides with the division of the cell genome. In addition, lentiviral vectors can effectively infect and integrate into non-dividing cells. The above characteristics make the lentiviral vector distinct from other viral vectors (such as non-integrated adenoviral vectors, adeno-associated viral vectors with low integration rates, and traditional retroviral vectors that only integrate dividing cells). A large number of literature studies have shown that tissues or cells that express the target gene for a long time mediated by lentiviral vectors include brain, liver, muscle, retina, hematopoietic stem cells, bone marrow mesenchymal stem cells, macrophages, etc.