All-in-One T7 Cap1 (3' OMe) mRNA Synthesis Kit

Name: All-in-One T7 Cap1 (3' OMe) mRNA Synthesis Kit
SKU: RSNK-009
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RSNK-009

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Product Information

Cat. No.	RSNK-009
Description	All-in-One T7 Cap1 (3' OMe) mRNA Synthesis Kit is designed for quick production of m7(3'OMeG)(5')ppp(5')(2'OMeA)pG capped and poly(A) tailed mRNA in vitro. RNA synthesized using this kit can be used in a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, et al.
Applications	• In vitro translation • Aantisense RNA and RNAi experiments • RNA vaccines • RNA structure and function studies
Size	25 rxns
Storage	Store at -20°C.

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Customer Q&As

What is 3' OMe?

A: 3' OMe refers to the 3' end modification of a nucleic acid molecule, typically RNA, with a methyl group (-CH3) at the 3' hydroxyl (-OH) position of the ribose sugar.

How to implement the 3' OMe modification?

A: This is accomplished through a chemical synthesis method called solid-phase synthesis, where the 3' end of the RNA is modified with a 3'-O-levulinyl protecting group during oligonucleotide synthesis. After the RNA synthesis is completed, the 3'-O-levulinyl protecting group is removed, exposing the 3'-OH group, which can then be methylated using a methylating agent such as iodomethane.

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Customer Reviews

Worked Very Well

Great kit. T7 Cap1 (3' OMe) mRNA Synthesis Kit can produce large amounts of Cap 1- and N1-Methylpseudouridine-modified (m)RNA via in vitro transcription with T7 RNA polymerase.

United States

2021-01-02

Convenient

The resulting 5’-capped (m)RNA can subsequently be used for microinjection, transfection or in vitro translation experiments. All-in-One T7 Cap1 (3' OMe) mRNA Synthesis Kit makes the operation more convenient.

United States

2022-03-25

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All-in-One T7 Cap1 (3' OMe) mRNA Synthesis Kit

Product Information

Q & A

Customer Reviews

What is 3' OMe?

How to implement the 3' OMe modification?

Worked Very Well

Convenient

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