Panoply™ Human PTCH1 Over-expressing Stable Cell Line

Name: Panoply™ Human PTCH1 Over-expressing Stable Cell Line
SKU: CSC-SC012642
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012642

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC012642
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PTCH1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PTCH1 patched 1 [ Homo sapiens ]
Gene Symbol	PTCH1
Synonyms	PTC; BCNS; HPE7; PTC1; PTCH; NBCCS; PTCH11
Gene Description	patched homolog 1 (Drosophila)
Gene ID	5727
Uni Prot ID	Q13635
m RNA Refseq	NM_000264.3
Protein Refseq	NP_000255.2
Chromosome Location	9q22.3
Function	cholesterol binding; cyclin binding; hedgehog family protein binding; hedgehog receptor activity; heparin binding; patched binding; protein binding; protein complex binding; smoothened binding;
Pathway	Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Glypican 3 network, organism-specific biosystem; Hedgehog Signaling Pathway, organism-specific biosystem;
MIM	601309

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