Panoply™ Human PPARA Over-expressing Stable Cell Line

Name: Panoply™ Human PPARA Over-expressing Stable Cell Line
SKU: CSC-SC012171
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012171

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC012171
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	PPARA
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PPARA peroxisome proliferator-activated receptor alpha [ Homo sapiens ]
Gene Symbol	PPARA
Synonyms	PPAR; NR1C1; hPPAR; PPARalpha
Gene Description	peroxisome proliferator-activated receptor alpha
GeneID	5465
Uni ProtID	F1D8S4
mRNA Refseq	NM_001001928.2
Protein Refseq	NP_001001928.1
Chromosome Location	22q13.31
Function	DNA binding; RNA polymerase II repressing transcription factor binding; drug binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; lipid binding; protein binding; protein domain specific binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; ubiquitin conjugating enzyme binding; zinc ion binding;
Pathway	Activation of Gene Expression by SREBP (SREBF), organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem;
MIM	170998

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