Cat.No. : CSC-RO0200 Host Cell: CHO-K1
| Cat. No. | CSC-RO0200 |
| Description | This cell line is engineered to stably overexpress the monkey CD47 in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of monkey CD47 molecule (CD47) into CHO-K1, followed by stable cell selection. The expression of CD47 has been analyzed through flow cytometry. |
| Gene | CD47 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Treatment of Diffuse Large B Cell Lymphoma (DLBCL) patients using rituximab and the CHOP regimen commonly encounters challenges due to both inherent and acquired resistance mechanisms. The researchers investigated the novel bispecific fusion protein DSP107 for treating DLBCL. DSP107 targets the CD47:SIRPα signaling axis to enhance phagocytosis by macrophages and trogocytosis by PMNs against DLBCL cells. It also activates 4-1BB-mediated costimulatory signaling in T cells, boosting their anticancer activity. In vitro and in vivo studies demonstrated DSP107's ability to significantly reduce cancer cell numbers and tumor size, particularly when combined with rituximab. Safety evaluations in cynomolgus monkeys showed a favorable profile. These findings suggest that DSP107 is a promising therapeutic strategy for enhancing innate and adaptive immune responses in DLBCL treatment.
Figure 1. The researchers demonstrated that DSP107-mediated activation of T cell costimulatory signaling through 4-1BB requires specific binding to CD47, evidenced by increased IL-8 secretion upon cross-linking. (Cendrowicz E, et al., 2022)
Creative Biogene offers the Monkey CD47 Stable Cell Line—CHO-K1, designed for applications in cancer research and immunotherapy. This cell line can effectively explore CD47 interactions in immune response studies.
A: Our monkey CD47 stable cell line has been tested for long-term stability and maintains stable expression levels through multiple freezes and thaws. We regularly validate cell lines and record cell line stability data. Customers can request this data from us to understand the stability performance of our products.
A: Our monkey CD47 stable cell line has stable expression levels and has been widely used in research related to immune evasion. We provide detailed experimental protocols, including recommended experimental steps and technical tips, to help customers use our products in their research.
A: Our monkey CD47 stable cell line has been validated in multiple cell lines and shows good expression and stability in different cell types. Although it is mainly used in CHO-K1 cell line, it also shows good results in other cell lines, such as HEK293, NIH/3T3, etc. We provide relevant literature support that customers can refer to to understand the application of our products in different cell lines.
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CHO-K1 cells are ideal for biotechnology and biomedical research due to their efficient growth and protein expression capabilities. The cells provided by the supplier were in good shape without any abnormalities.
The gene expression of CD47 is stable, ensuring the accuracy of experimental data. The platform provides detailed instructions and technical support, making the entire user experience very professional and smooth. This product and supplier's service was impeccable and highly recommended.
I used Monkey CD47 Stable Cell Line - CHO-K1 for signal transduction studies with excellent results. The cells grew rapidly in the culture medium and maintained good shape and vitality. Gene expression analysis showed that the expression of CD47 is very stable, which provides reliable data support for studying the regulation of calcium concentration after cells bind to the extracellular matrix.
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