Cat.No. : CSC-RO0010 Host Cell: CHO-K1
| Cat. No. | CSC-RO0010 |
| Description | This cell line is engineered to stably overexpress the human SPATA2 in CHO-K1 cells. |
| Background | Spermatogenesis-associated protein 2 is a protein that in humans is encoded by the SPATA2 gene. This protein may have a role in the regulation of spermatogenesis. Alternative splicing results in different isoforms. |
| Gene | SPATA2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | SPATA2 spermatogenesis associated 2 [ Homo sapiens ] |
| Gene Symbol | SPATA2 |
| Synonyms | SPATA2; spermatogenesis associated 2; spermatogenesis-associated protein 2; KIAA0757; PD1; tamo; spermatogenesis associated PD1; spermatogenesis-associated protein PD1; FLJ13167; |
| Gene ID | 9825 |
| UniProt ID | Q9UM82 |
| mRNA Refseq | BC009481 |
| Chromosome Location | 20q13.13 |
| Function | molecular_function; |
| MIM | 607662 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human SPATA2 Stable Cell Line-CHO has potential applications in the study of neurodegenerative diseases. As SPATA2 may play a role in regulating neuronal apoptosis, this cell line can be used to study the role of SPATA2 in neurodegenerative diseases like Alzheimer's and potential therapeutic targets.
A: When conducting gene editing experiments with the Human SPATA2 Stable Cell Line-CHO, it is important to maintain the expression and function of the SPATA2 protein. The choice and design of gene editing tools should ensure specificity to the target gene and accurate validation of the editing effects.
A: The Human SPATA2 Stable Cell Line-CHO has promising prospects in cancer therapy research. Due to the role of SPATA2 in regulating apoptosis, this cell line can be used to study the role of SPATA2 in cancer progression and develop novel anti-cancer therapies targeting SPATA2.
A: When conducting drug screening with the Human SPATA2 Stable Cell Line-CHO, experiments should be designed to evaluate the effects of drugs on the SPATA2-mediated apoptotic pathway. The screening and assessment of potential drug candidates can be performed by measuring cell viability, apoptotic markers, and changes in SPATA2 activity.
A: By using the Human SPATA2 Stable Cell Line-CHO, co-immunoprecipitation and mass spectrometry can be employed to study the interactions of SPATA2 with other proteins, revealing its role in cellular signaling. Additionally, techniques such as bimolecular fluorescence complementation can be used to further validate these interactions.
A: The Human SPATA2 Stable Cell Line-CHO, due to its stable expression of human SPATA2 protein, holds significant potential in apoptosis research. SPATA2 plays a crucial role in regulating the process of apoptosis, especially in the death receptor pathway. This cell line can be used to study the specific mechanisms of SPATA2 in apoptotic signaling pathways.
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