Human SPATA2 Stable Cell Line-CHO-K1

Name: Human SPATA2 Stable Cell Line-CHO-K1
SKU: CSC-RO0010
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0010

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0010
Description	This cell line is engineered to stably overexpress the human SPATA2 in CHO-K1 cells.
Background	Spermatogenesis-associated protein 2 is a protein that in humans is encoded by the SPATA2 gene. This protein may have a role in the regulation of spermatogenesis. Alternative splicing results in different isoforms.
Target Gene	SPATA2
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SPATA2 spermatogenesis associated 2 [ Homo sapiens ]
Gene Symbol	SPATA2
Synonyms	SPATA2; spermatogenesis associated 2; spermatogenesis-associated protein 2; KIAA0757; PD1; tamo; spermatogenesis associated PD1; spermatogenesis-associated protein PD1; FLJ13167;
GeneID	9825
Uni ProtID	Q9UM82
mRNA Refseq	BC009481
Chromosome Location	20q13.13
Function	molecular_function;
MIM	607662

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the potential application of the Human SPATA2 Stable Cell Line-CHO in studying neurodegenerative diseases?

A: The Human SPATA2 Stable Cell Line-CHO has potential applications in the study of neurodegenerative diseases. As SPATA2 may play a role in regulating neuronal apoptosis, this cell line can be used to study the role of SPATA2 in neurodegenerative diseases like Alzheimer's and potential therapeutic targets.

What key factors should be considered when conducting gene editing experiments with the Human SPATA2 Stable Cell Line-CHO?

A: When conducting gene editing experiments with the Human SPATA2 Stable Cell Line-CHO, it is important to maintain the expression and function of the SPATA2 protein. The choice and design of gene editing tools should ensure specificity to the target gene and accurate validation of the editing effects.

What are the prospects of the Human SPATA2 Stable Cell Line-CHO in cancer therapy research?

A: The Human SPATA2 Stable Cell Line-CHO has promising prospects in cancer therapy research. Due to the role of SPATA2 in regulating apoptosis, this cell line can be used to study the role of SPATA2 in cancer progression and develop novel anti-cancer therapies targeting SPATA2.

How should experiments be designed for drug screening using the Human SPATA2 Stable Cell Line-CHO?

A: When conducting drug screening with the Human SPATA2 Stable Cell Line-CHO, experiments should be designed to evaluate the effects of drugs on the SPATA2-mediated apoptotic pathway. The screening and assessment of potential drug candidates can be performed by measuring cell viability, apoptotic markers, and changes in SPATA2 activity.

How can the Human SPATA2 Stable Cell Line-CHO be used to study the protein interactions of SPATA2?

A: By using the Human SPATA2 Stable Cell Line-CHO, co-immunoprecipitation and mass spectrometry can be employed to study the interactions of SPATA2 with other proteins, revealing its role in cellular signaling. Additionally, techniques such as bimolecular fluorescence complementation can be used to further validate these interactions.

What is the potential application of the Human SPATA2 Stable Cell Line-CHO in apoptosis research?

A: The Human SPATA2 Stable Cell Line-CHO, due to its stable expression of human SPATA2 protein, holds significant potential in apoptosis research. SPATA2 plays a crucial role in regulating the process of apoptosis, especially in the death receptor pathway. This cell line can be used to study the specific mechanisms of SPATA2 in apoptotic signaling pathways.

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