Human SLAMF1 Stable Cell Line-HEK293T

Name: Human SLAMF1 Stable Cell Line-HEK293T
SKU: CSC-RO0115
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0115

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0115
Description	HEK293T-SLAMF1 cell line is a stably transfected cell line which expresses human signaling lymphocytic activation molecule family member 1 (SLAMF1).
Target Gene	SLAMF1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SLAMF1 signaling lymphocytic activation molecule family member 1 [ Homo sapiens ]
Gene Symbol	SLAMF1
Synonyms	SLAMF1; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule , SLAM; signaling lymphocytic activation molecule; CD150; IPO-3; SLAM; CDw150;
Gene ID	6504
Uni Prot ID	Q13291
m RNA Refseq	BC012602
Chromosome Location	1q23.3
Function	antigen binding; receptor activity; transmembrane signaling receptor activity;
Pathway	Measles, organism-specific biosystem; Measles, conserved biosystem;
MIM	603492

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Customer Reviews

Customer Q&As

What is the genetic modification involved in the creation of the Human SLAMF1 Stable Cell Line-HEK293T?

A: The Human SLAMF1 Stable Cell Line-HEK293T is engineered to express the SLAMF1 gene stably, typically achieved by introducing a vector containing the SLAMF1 gene into HEK293T cells.

How does the Human SLAMF1 Stable Cell Line-HEK293T contribute to the study of immune cell signaling?

A: This cell line is valuable for investigating the role of SLAMF1 in immune cell activation and signaling, as SLAMF1 is a member of the signaling lymphocytic activation molecule (SLAM) family.

What are the key phenotypic characteristics of the Human SLAMF1 Stable Cell Line-HEK293T?

A: The cell line exhibits stable expression of the SLAMF1 protein, which can be confirmed through various assays such as Western blotting and flow cytometry.

How should the Human SLAMF1 Stable Cell Line-HEK293T be stored and revived for long-term use?

A: The cell line should be cryopreserved in a suitable freezing medium and stored in liquid nitrogen. For revival, cells should be thawed quickly and transferred to a 37°C incubator.

How can the Human SLAMF1 Stable Cell Line-HEK293T be used in drug screening and development?

A: The cell line can be utilized for high-throughput screening of compounds that modulate SLAMF1 activity, aiding in the identification of potential therapeutic agents.

How does the Human SLAMF1 Stable Cell Line-HEK293T facilitate the investigation of SLAMF1's role in immune cell differentiation?

A: By providing a model system with stable SLAMF1 expression, researchers can investigate how SLAMF1 influences the differentiation of various immune cell types.

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