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Cat.No. : CSC-RO0115 Host Cell: HEK293T
| Cat. No. | CSC-RO0115 |
| Description | HEK293T-SLAMF1 cell line is a stably transfected cell line which expresses human signaling lymphocytic activation molecule family member 1 (SLAMF1). |
| Gene | SLAMF1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human SLAMF1 Stable Cell Line-HEK293T is engineered to express the SLAMF1 gene stably, typically achieved by introducing a vector containing the SLAMF1 gene into HEK293T cells.
A: This cell line is valuable for investigating the role of SLAMF1 in immune cell activation and signaling, as SLAMF1 is a member of the signaling lymphocytic activation molecule (SLAM) family.
A: The cell line exhibits stable expression of the SLAMF1 protein, which can be confirmed through various assays such as Western blotting and flow cytometry.
A: The cell line should be cryopreserved in a suitable freezing medium and stored in liquid nitrogen. For revival, cells should be thawed quickly and transferred to a 37°C incubator.
A: The cell line can be utilized for high-throughput screening of compounds that modulate SLAMF1 activity, aiding in the identification of potential therapeutic agents.
A: By providing a model system with stable SLAMF1 expression, researchers can investigate how SLAMF1 influences the differentiation of various immune cell types.
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