Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1589 Host Cell : HEK293
| Cat. No. | CLOE-1589 |
| Introduction | This cell lysate over-expresses the extracellular domain of human LDLR (NP_000518.1) (Met 1-Arg 788) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | LDLR |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | LDLR low density lipoprotein receptor [ Homo sapiens ] |
| Gene Symbol | LDLR |
| Synonyms | FH; FHC; LDLCQ2 |
| Gene ID | 3949 |
| Uni Prot ID | P01130 |
| m RNA Refseq | NM_000527.4 |
| Protein Refseq | NP_000518.1 |
| Chromosome Location | 19p13.2 |
| Function | calcium ion binding; glycoprotein binding; low-density lipoprotein receptor activity; protein binding; very-low-density lipoprotein particle receptor activity; |
| Pathway | Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Hepatitis C, organism-specific biosystem; |
| MIM | 606945 |
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