Human LDLR KO Cell Line-HeLa

Name: Human LDLR KO Cell Line-HeLa
SKU: CSC-RT2986
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2986

Target Gene : LDLR Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2986
Description	This cell is a stable cell line with a homozygous knockout of human LDLR using CRISPR/Cas9
Target Gene	LDLR
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LDLR low density lipoprotein receptor [ Homo sapiens ]
Gene Symbol	LDLR
Synonyms	FH; FHC; LDLCQ2
Gene ID	3949
Uni Prot ID	P01130
m RNA Refseq	NM_000527.4
Protein Refseq	NP_000518.1
Chromosome Location	19p13.2
Function	calcium ion binding; glycoprotein binding; low-density lipoprotein receptor activity; protein binding; very-low-density lipoprotein particle receptor activity;
Pathway	Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Hepatitis C, organism-specific biosystem;
MIM	606945

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