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Cat.No. : CSC-RO0227 Host Cell: CHO-K1
| Cat. No. | CSC-RO0227 |
| Description | This cell line is engineered to stably overexpress the human ITGAE in CHO-K1 cells. |
| Introduction | CHO-K1/ITGAE is clonally derived from CHO-K1 cell line and stably expresses human integrin subunit alpha E (ITGAE). The expression of CLEC4C in this cell line has been validated through flow cytometry. |
| Gene | ITGAE |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The CHO-K1 cell line is a Chinese Hamster Ovary (CHO) cell line that is widely used for recombinant protein expression. Its advantage over other cell lines is its ability to perform complex post-translational modifications, which are crucial for the proper folding and function of proteins like ITGAE. Additionally, CHO-K1 cells are known for their high stability and reproducibility, making them ideal for stable cell line generation.
A: Establishing a stable cell line expressing ITGAE in CHO-K1 cells can be challenging due to the need for efficient gene transfer, selection, and validation. Challenges include optimizing transfection conditions, developing appropriate selection markers, and ensuring the stable and functional expression of ITGAE without affecting cell viability or growth.
A: The cell line can be used in high-throughput screening assays to identify compounds that modulate ITGAE activity, which could potentially lead to the development of new allergy treatments. By testing the effects of various compounds on ITGAE signaling pathways, researchers can identify potential drug candidates that could inhibit allergic responses.
A: The cell line allows researchers to model the cellular responses to IgE, which is central to allergic diseases. By studying the ITGAE signaling pathway in this context, scientists can better understand the molecular mechanisms underlying allergic reactions and develop targeted therapies.
A: Maintaining the stability of the cell line over long-term culture requires careful monitoring of cell growth, selection pressure, and ITGAE expression levels. Regular testing for mycoplasma contamination and karyotype analysis are also essential to ensure the genetic integrity of the cell line.
A: The cell line can contribute to personalized medicine by helping researchers understand the variability in ITGAE expression and function among individuals. This knowledge can be used to develop targeted therapies that take into account individual genetic and molecular profiles, leading to more effective treatments for allergic patients.
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