Human ITGAE Stable Cell Line - CHO-K1

Name: Human ITGAE Stable Cell Line - CHO-K1
SKU: CSC-RO0227
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0227

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0227
Description	This cell line is engineered to stably overexpress the human ITGAE in CHO-K1 cells.
Introduction	CHO-K1/ITGAE is clonally derived from CHO-K1 cell line and stably expresses human integrin subunit alpha E (ITGAE). The expression of CLEC4C in this cell line has been validated through flow cytometry.
Target Gene	ITGAE
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ITGAE integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) [ Homo sapiens ]
Gene Symbol	ITGAE
Synonyms	CD103; HUMINAE
Gene Description	integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
Gene ID	3682
Uni Prot ID	P38570
m RNA Refseq	NM_002208.4
Protein Refseq	NP_002199.3
Chromosome Location	17p13
Pathway	E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Integrin cell surface interactions, organism-specific biosystem; Integrin-mediated cell adhesion, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; Signal Transduction, organism-specific biosystem;
MIM	604682

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Q & A

Customer Reviews

Customer Q&As

How does the CHO-K1 cell line differ from other cell lines used for ITGAE expression?

A: The CHO-K1 cell line is a Chinese Hamster Ovary (CHO) cell line that is widely used for recombinant protein expression. Its advantage over other cell lines is its ability to perform complex post-translational modifications, which are crucial for the proper folding and function of proteins like ITGAE. Additionally, CHO-K1 cells are known for their high stability and reproducibility, making them ideal for stable cell line generation.

What are the challenges in establishing a Human ITGAE Stable Cell Line in CHO-K1 cells?

A: Establishing a stable cell line expressing ITGAE in CHO-K1 cells can be challenging due to the need for efficient gene transfer, selection, and validation. Challenges include optimizing transfection conditions, developing appropriate selection markers, and ensuring the stable and functional expression of ITGAE without affecting cell viability or growth.

How can the Human ITGAE Stable Cell Line - CHO-K1 be utilized in drug screening for allergy treatments?

A: The cell line can be used in high-throughput screening assays to identify compounds that modulate ITGAE activity, which could potentially lead to the development of new allergy treatments. By testing the effects of various compounds on ITGAE signaling pathways, researchers can identify potential drug candidates that could inhibit allergic responses.

How does the Human ITGAE Stable Cell Line - CHO-K1 contribute to the understanding of IgE-mediated allergic diseases?

A: The cell line allows researchers to model the cellular responses to IgE, which is central to allergic diseases. By studying the ITGAE signaling pathway in this context, scientists can better understand the molecular mechanisms underlying allergic reactions and develop targeted therapies.

What are the considerations for maintaining the stability of the Human ITGAE Stable Cell Line - CHO-K1 over long-term culture?

A: Maintaining the stability of the cell line over long-term culture requires careful monitoring of cell growth, selection pressure, and ITGAE expression levels. Regular testing for mycoplasma contamination and karyotype analysis are also essential to ensure the genetic integrity of the cell line.

What is the role of the Human ITGAE Stable Cell Line - CHO-K1 in the development of personalized medicine for allergic patients?

A: The cell line can contribute to personalized medicine by helping researchers understand the variability in ITGAE expression and function among individuals. This knowledge can be used to develop targeted therapies that take into account individual genetic and molecular profiles, leading to more effective treatments for allergic patients.

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