Human CD200R1 Stable Cell Line-CHO-K1

Name: Human CD200R1 Stable Cell Line-CHO-K1
SKU: CSC-RO0005
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0005

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0005
Description	This cell line is engineered to stably overexpress the human CD200R1 in CHO-K1 cells.
Background	This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants.
Target Gene	CD200R1
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD200R1 CD200 receptor 1 [ Homo sapiens ]
Gene Symbol	CD200R1
Synonyms	OX2R; MOX2R; CD200R; HCRTR2
Gene Description	CD200 receptor 1
GeneID	131450
Uni ProtID	Q8TD46
mRNA Refseq	NM_138940.2
Protein Refseq	NP_620386.1
Chromosome Location	3q13.2
Function	protein binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem;
MIM	607546

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How can the Human CD200R1 Stable Cell Line-CHO be used to study the interaction between CD200R1 and its ligand CD200?

A: By using the Human CD200R1 Stable Cell Line-CHO, co-immunoprecipitation experiments can be conducted to study the interaction between CD200R1 and its natural ligand CD200. Additionally, cell binding experiments and flow cytometry can be used to assess the binding affinity and specificity of CD200R1 to CD200.

How should experiments be designed for drug screening using the Human CD200R1 Stable Cell Line-CHO?

A: For drug screening, experiments should be designed to evaluate the binding capacity and functional impact of different compounds on the CD200R1 protein in the Human CD200R1 Stable Cell Line-CHO. ELISA, flow cytometry, or functional cell assays can be used to assess the efficiency of antibody or small molecule drug binding and the modulation of the CD200R1 signaling pathway.

What is the potential application of the Human CD200R1 Stable Cell Line-CHO in studying neurodegenerative diseases?

A: The Human CD200R1 Stable Cell Line-CHO has potential applications in the study of neurodegenerative diseases. As CD200R1 plays an important role in regulating immune responses in the central nervous system, this cell line can be used to study the mechanisms of CD200R1 in neurodegenerative diseases and potential therapeutic targets.

What key factors should be considered when conducting gene editing experiments with the Human CD200R1 Stable Cell Line-CHO?

A: When conducting gene editing experiments with the Human CD200R1 Stable Cell Line-CHO, it is important to maintain the expression and function of the CD200R1 protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.

What are the prospects of the Human CD200R1 Stable Cell Line-CHO in studying tumor immune escape mechanisms?

A: The Human CD200R1 Stable Cell Line-CHO has promising prospects in studying tumor immune escape mechanisms. As CD200R1 is associated with tumor cell immune evasion, this cell line can be used to study the role of CD200R1 in the tumor microenvironment and immunotherapeutic strategies targeting CD200R1.

What is the potential application of the Human CD200R1 Stable Cell Line-CHO in immune regulation research?

A: The Human CD200R1 Stable Cell Line-CHO, due to its stable expression of human CD200 receptor 1 (CD200R1), has significant potential in immune regulation research. CD200R1, as an immunosuppressive receptor, plays a vital role in regulating immune responses and maintaining immune tolerance. This cell line can be used to study the specific roles and regulatory mechanisms of CD200R1 in the immune system.

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