Cat.No. : CSC-RO0005 Host Cell: CHO-K1
| Cat. No. | CSC-RO0005 |
| Description | This cell line is engineered to stably overexpress the human CD200R1 in CHO-K1 cells. |
| Background | This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. |
| Gene | CD200R1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CD200R1 CD200 receptor 1 [ Homo sapiens ] |
| Gene Symbol | CD200R1 |
| Synonyms | OX2R; MOX2R; CD200R; HCRTR2 |
| Gene Description | CD200 receptor 1 |
| Gene ID | 131450 |
| UniProt ID | Q8TD46 |
| mRNA Refseq | NM_138940.2 |
| Protein Refseq | NP_620386.1 |
| Chromosome Location | 3q13.2 |
| Function | protein binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; |
| MIM | 607546 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: By using the Human CD200R1 Stable Cell Line-CHO, co-immunoprecipitation experiments can be conducted to study the interaction between CD200R1 and its natural ligand CD200. Additionally, cell binding experiments and flow cytometry can be used to assess the binding affinity and specificity of CD200R1 to CD200.
A: For drug screening, experiments should be designed to evaluate the binding capacity and functional impact of different compounds on the CD200R1 protein in the Human CD200R1 Stable Cell Line-CHO. ELISA, flow cytometry, or functional cell assays can be used to assess the efficiency of antibody or small molecule drug binding and the modulation of the CD200R1 signaling pathway.
A: The Human CD200R1 Stable Cell Line-CHO has potential applications in the study of neurodegenerative diseases. As CD200R1 plays an important role in regulating immune responses in the central nervous system, this cell line can be used to study the mechanisms of CD200R1 in neurodegenerative diseases and potential therapeutic targets.
A: When conducting gene editing experiments with the Human CD200R1 Stable Cell Line-CHO, it is important to maintain the expression and function of the CD200R1 protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.
A: The Human CD200R1 Stable Cell Line-CHO has promising prospects in studying tumor immune escape mechanisms. As CD200R1 is associated with tumor cell immune evasion, this cell line can be used to study the role of CD200R1 in the tumor microenvironment and immunotherapeutic strategies targeting CD200R1.
A: The Human CD200R1 Stable Cell Line-CHO, due to its stable expression of human CD200 receptor 1 (CD200R1), has significant potential in immune regulation research. CD200R1, as an immunosuppressive receptor, plays a vital role in regulating immune responses and maintaining immune tolerance. This cell line can be used to study the specific roles and regulatory mechanisms of CD200R1 in the immune system.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
