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BsuRI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0309
Reaction Buffer
10mM Tris-HCl (pH8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
CG↓CC
Size/Form
3000 Units;6000 Units
Storage
10mM Tris-HCl (pH7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What are the optimal reaction conditions for BsuRI?

A: The best cutting performance is observed at 37°C in R buffer.

Is BsuRI sensitive to methylation?

A: It is not sensitive to Dam, Dcm, or CpG methylation.

Is a buffer included with BsuRI?

A: Restriction endonucleases are shipped with their optimal digestion buffer or can be purchased separately.

What are the shipping conditions for BsuRI?

A: They are shipped with dry ice, which may result in ice formation on the dry ice, but it does not affect product quality.

Why is BsuRI enzyme digestion failing?

A: The main reason for the failure of DNA cleavage reactions is the presence of contaminants or inhibitors in the template DNA (e.g., phenol, chloroform, detergents, ethanol, excess salts, EDTA, etc.).

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