Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0309
| Cat. No. | EROT0309 |
| Recognition Site | CG↓CC |
| Size | 3000 Units;6000 Units |
| Reaction Buffer | 10mM Tris-HCl (pH8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: The best cutting performance is observed at 37°C in R buffer.
A: It is not sensitive to Dam, Dcm, or CpG methylation.
A: Restriction endonucleases are shipped with their optimal digestion buffer or can be purchased separately.
A: They are shipped with dry ice, which may result in ice formation on the dry ice, but it does not affect product quality.
A: The main reason for the failure of DNA cleavage reactions is the presence of contaminants or inhibitors in the template DNA (e.g., phenol, chloroform, detergents, ethanol, excess salts, EDTA, etc.).
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