Cat.No. : LVIM002Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LVIM002Z |
| Description | Lentivirus expressing untagged SV40 Small T and Large T Antigen under the control of EF1α promoter, and puromycin resistance marker for mammalian cell selection. |
| Target Gene | Small/Large T Antigen |
| Species | SV40 |
| Application | SV40 small/large T antigen lentivirus (EF1α, Puro) is a versatile tool used primarily in molecular and cell biology research. Here are some of its key applications: Cell line immortalization: SV40 large T antigen is able to inactivate tumor suppressor proteins such as p53 and retinoblastoma protein (pRb), allowing cells to bypass normal cellular senescence and continue to divide, effectively immortalizing primary cells. This is very useful for creating stable, long-term cell cultures for research. Gene expression studies: The use of the EF1α promoter ensures that SV40 small/large T antigen is stably and consistently expressed in a variety of cell types, facilitating gene regulation and expression studies under controlled conditions. Cancer research: Since SV40 large T antigen is known to interfere with tumor suppressor pathways, it can be used to create models to study cancer biology, tumorigenesis, and the underlying mechanisms of cell cycle regulation and apoptosis. Drug screening and development: Immortalized cell lines generated using SV40 T antigen can be used in high-throughput screening assays to evaluate the efficacy and toxicity of potential therapeutic compounds. Pluripotent stem cell research: SV40 T antigen helps maintain the undifferentiated state of pluripotent stem cells, which is critical for stem cell research and regenerative medicine. |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: The concentration must be optimized case-by-case and is cell type dependent. On average, typical selection amounts are around 0.1 to 0.5 ug per ml.
A: Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations than those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will select for the population of transduced cells you wish to select for, without going below the minimum antibiotic concentration you have established in your killing curve.
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Allows selection by puromycin antibiotics and the transduced cells are used for long-term expression, which is very convenient and good to use.
The SV40 large T antigen in this product leads to cell immortalization and allows for high titer lentivirus production in cell lines.
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