Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : PMRN-0305
| Cat. No. | PMRN-0305 |
| Description | SpCas9 mRNA expresses the Cas9 protein with two nuclear localization signals (NLS) and a C terminal HA epitope tag that enhance the rate of DNA cleavage and facilitate downstream purification. It can be used with a purified gRNA sequence for genomic DNA target recognition and cleavage in the CRISPR-Cas9 genome editing system. Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription to generate capped transcripts. ARCA obtains the ability only to insert in the proper orientation which ensures ARCA-capped mRNA with high translation efficacy. Meanwhile, the incorporation of 5-Methyl-CTP and ψUTP can reduce the immunogenicity of the resulting mRNA. |
| Features |
• mRNA synthesized on error free sequence verified plasmid DNA template • ARCA capping and poly-A tail incorporated • mRNA modified with 5-Methyl-CTP and ψUTP • Degrades the DNA template after RNA synthesis with DNase |
| Storage | Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers. |
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