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CC-1250

Rgs10 Easy KO Kit (Mouse)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-1250

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Product Information

Gene Information

Cat. No. CC-1250
Description A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr Rgs10
Gene Name Rgs10 regulator of G-protein signalling 10 [ Mus musculus ]
Species Mouse
Ensembl ID ENSMUSG00000030844
NCBIGene ID 67865
Uni Prot ID Q9CQE5
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Applications This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions 5–10 reactions per target gene
Kit Components 2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
Gene Name Rgs10 regulator of G-protein signalling 10 [ Mus musculus ]
Gene Symbol Rgs10
Synonyms 2310010N19Rik
Gene Description regulator of G-protein signalling 10
Gene ID 67865
Uni Prot ID Q32MD7
m RNA Refseq NM_026418.2
Protein Refseq NP_080694.1
Chromosome Location 7 F3; 7
Pathway Calcium Regulation in the Cardiac Cell, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem;
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