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CC-1251

Rgs10 Easy KO Kit (Human)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-1251

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Product Information

Gene Information

Cat. No. CC-1251
Description A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr Rgs10
Gene Name RGS10 regulator of G-protein signaling 10 [ Homo sapiens ]
Species Human
Ensembl ID ENSMUSG00000030844
NCBIGene ID 67865
Uni Prot ID Q9CQE5
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Applications This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions 5–10 reactions per target gene
Kit Components 2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
Gene Name RGS10 regulator of G-protein signaling 10 [ Homo sapiens ]
Gene Symbol RGS10
Gene Description regulator of G-protein signalling 10
Gene ID 6001
Uni Prot ID O43665
m RNA Refseq NM_001005339.1
Protein Refseq NP_001005339.1
Chromosome Location 10q25
Pathway Calcium Regulation in the Cardiac Cell, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem;
MIM 602856
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