MTAP Easy KO Kit (Human)

Name: MTAP Easy KO Kit (Human)
SKU: CC-899
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-899

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Product Information

Gene Information

Cat. No.	CC-899
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Mtap
Gene Name	MTAP methylthioadenosine phosphorylase [ Homo sapiens ]
Species	Human
Ensembl ID	ENSMUSG00000062937
NCBIGene ID	66902
Uni Prot ID	Q9CQ65
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	MTAP methylthioadenosine phosphorylase [ Homo sapiens ]
Gene Symbol	MTAP
Synonyms	MTAP; methylthioadenosine phosphorylase; S-methyl-5-thioadenosine phosphorylase; c86fus; MSAP; MTAPase; MTA phosphorylase; MeSAdo phosphorylase; 5-methylthioadenosine phosphorylase;
Gene ID	4507
Uni Prot ID	Q13126
m RNA Refseq	BC018625
Chromosome Location	9p21
Function	S-methyl-5-thioadenosine phosphorylase activity; phosphorylase activity;
Pathway	Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem;
MIM	156540

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