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Mouse Cd40lg Stable Cell Line - NIH/3T3

Mouse Cd40lg Stable Cell Line - NIH/3T3

Cat.No. :  CSC-RO01395 Host Cell:  Mouse embryonic fibroblast cell line (NIH/3T3)

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Cat. No. CSC-RO01395
Description This cell line is engineered to stably express Mus musculus (Murine, House mouse) CD40 ligand (Cd40lg) in Mouse embryonic fibroblast cell line (NIH/3T3).
Gene Cd40lg
Gene Species Mus musculus (Murine, House mouse)
Host Cell Mouse embryonic fibroblast cell line (NIH/3T3)
Host Cell Species Mus musculus (Murine, House mouse) cell line
Stability This cell line is stable at least 10 passages.
Product Type Mouse gene overexpression stable cell line
Applications 1) investigation of gene function
2) screening and validation of antibodies
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) mycoplasma detection
Size Form One vial of frozen cells, typically >1x10^6cells/vial
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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