Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2089 Host Cell : HEK293
| Cat. No. | CLOE-2089 |
| Description | This cell lysate overexpresses rat Cd40lg (Gly115-Leu260) with the Fc region of human IgG1 at the N-terminus. Purification of target protein from this cell lysate has been verified. |
| Introduction | This cell lysate overexpresses rat Cd40lg (Gly115-Leu260) with the Fc region of human IgG1 at the N-terminus. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Cd40lg |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4℃ , or prepare aliquots and store at -80℃. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Cd40lg CD40 ligand [ Mus musculus ] |
| Gene Symbol | Cd40lg |
| Synonyms | IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5 |
| Gene Description | CD40 ligand |
| Gene ID | 21947 |
| Uni Prot ID | P27548 |
| m RNA Refseq | NM_011616.2 |
| Protein Refseq | NP_035746.2 |
| Chromosome Location | X A5; X 18.0 cM |
| Function | CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; |
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