Human CD40LG HEK293 Cell Lysate

Name: Human CD40LG HEK293 Cell Lysate
SKU: CLOE-1582
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1582 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1582
Introduction	This cell lysate over-expresses the human CD40LG (NP_000065.1) (Met 113-Leu 261) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CD40LG
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CD40LG CD40 ligand [ Homo sapiens ]
Gene Symbol	CD40LG
Synonyms	IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Gene Description	CD40 ligand (TNF superfamily, member 5, hyper-IgM syndrome)
Gene ID	959
Uni Prot ID	P29965
m RNA Refseq	NM_000074.2
Protein Refseq	NP_000065.1
Chromosome Location	Xq26
Function	CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem;
MIM	300386

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