Hyal2 Easy KO Kit (Human)

Name: Hyal2 Easy KO Kit (Human)
SKU: CC-665
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-665

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Product Information

Gene Information

Cat. No.	CC-665
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Hyal2
Gene Name	HYAL2 hyaluronoglucosaminidase 2 [ Homo sapiens ]
Species	Human
Ensembl ID	ENSMUSG00000010047
NCBIGene ID	15587
Uni Prot ID	O35632
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	HYAL2 hyaluronoglucosaminidase 2 [ Homo sapiens ]
Gene Symbol	HYAL2
Synonyms	LUCA2
Gene Description	hyaluronoglucosaminidase 2
Gene ID	8692
Uni Prot ID	Q12891
m RNA Refseq	NM_033158.4
Protein Refseq	NP_149348.2
Chromosome Location	3p21.3
Function	enzyme binding; hyaluronic acid binding; hyaluronoglucuronidase activity; hyalurononglucosaminidase activity; NOT hyalurononglucosaminidase activity; hyalurononglucosaminidase activity; protein binding; receptor signaling protein tyrosine kinase inhibitor activity; receptor tyrosine kinase binding; transforming growth factor beta binding; viral receptor activity;
Pathway	Chondroitin sulfate degradation, organism-specific biosystem; Chondroitin sulfate degradation, conserved biosystem; Dermatan sulfate degradation, organism-specific biosystem; Dermatan sulfate degradation, conserved biosystem; Disease, organism-specific biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem;
MIM	603551

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