Hyal2 Easy KO Kit (Mouse)

Name: Hyal2 Easy KO Kit (Mouse)
SKU: CC-664
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-664

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Product Information

Gene Information

Cat. No.	CC-664
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Hyal2
Gene Name	Hyal2 hyaluronoglucosaminidase 2 [ Mus musculus ]
Species	Mouse
Ensembl ID	ENSMUSG00000010047
NCBIGene ID	15587
Uni Prot ID	O35632
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	Hyal2 hyaluronoglucosaminidase 2 [ Mus musculus ]
Gene Symbol	Hyal2
Synonyms	AI256841; AU020858; AW555733
Gene Description	hyaluronoglucosaminidase 2
Gene ID	15587
Uni Prot ID	O35632
m RNA Refseq	NM_010489.2
Protein Refseq	NP_034619.2
Chromosome Location	9 F1; 9 60.0 cM
Function	contributes_to RNA polymerase II transcription coactivator activity; catalytic activity; enzyme binding; enzyme binding; hyaluronic acid binding; hyaluronic acid binding; hyaluronoglucuronidase activity; hyalurononglucosaminidase activity; hyalurononglucosaminidase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; receptor signaling protein tyrosine kinase inhibitor activity; receptor tyrosine kinase binding; transforming growth factor beta binding; viral receptor activity; NOT viral receptor activity; viral receptor activity;
Pathway	Chondroitin sulfate degradation, organism-specific biosystem; Chondroitin sulfate degradation, conserved biosystem; Dermatan sulfate degradation, organism-specific biosystem; Dermatan sulfate degradation, conserved biosystem; Disease, organism-specific biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem;

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