Human VCL KO Cell Line-Hela

Name: Human VCL KO Cell Line-Hela
SKU: CSC-RT2849
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2849

Target Gene : VCL Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2849
Description	This cell is a stable cell line with a homozygous knockout of human VCL using CRISPR/Cas9.
Target Gene	VCL
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	VCL vinculin [ Homo sapiens ]
Gene Symbol	VCL
Synonyms	MVCL; CMD1W; CMH15
Gene Description	vinculin
GeneID	7414
Uni ProtID	B3KXA2
mRNA Refseq	NM_003373.3
Protein Refseq	NP_003364.1
Chromosome Location	10q22.2
Function	Rho GTPase binding; NOT actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; dystroglycan binding; protein binding; structural molecule activity;
Pathway	Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Focal Adhesion, organism-specific biosystem;
MIM	193065

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