Human VCL HEK293 Cell Lysate

Name: Human VCL HEK293 Cell Lysate
SKU: CLOE-1792
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1792 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1792
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human VCL (P18206-2) (Met 1-Gln 1066). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	VCL
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	VCL vinculin [ Homo sapiens ]
Gene Symbol	VCL
Synonyms	MVCL; CMD1W; CMH15
Gene Description	vinculin
GeneID	7414
Uni ProtID	B3KXA2
mRNA Refseq	NM_003373.3
Protein Refseq	NP_003364.1
Chromosome Location	10q22.2
Function	Rho GTPase binding; NOT actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; dystroglycan binding; protein binding; structural molecule activity;
Pathway	Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Focal Adhesion, organism-specific biosystem;
MIM	193065

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