Mouse Vcl HEK293 Cell Lysate

Name: Mouse Vcl HEK293 Cell Lysate
SKU: CLOE-2458
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2458 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2458
Introduction	This cell lysate over-expresses mouse Vcl (NP_033528.3) (Met1-Gln1066) fused with C-terminal his tag. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	Vcl
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	Vcl vinculin [ Mus musculus ]
Gene Symbol	Vcl
Synonyms	AA571387; AI462105; AW545629; 9430097D22
Gene Description	vinculin
GeneID	22330
Uni ProtID	Q64727
mRNA Refseq	NM_009502.4
Protein Refseq	NP_033528.3
Chromosome Location	14 A3; 14 2.5 cM
Function	Rho GTPase binding; actin binding; alpha-catenin binding; dystroglycan binding; protein binding; structural molecule activity;
Pathway	Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Focal Adhesion, organism-specific biosystem;

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