Cat.No. : CSC-RO01324 Host Cell: Human colorectal carcinoma / colon cancer cell line (HCT116)
| Cat. No. | CSC-RO01324 |
| Description | This cell line is engineered to stably express Homo sapiens (human) sequestosome 1 (SQSTM1) in Human colorectal carcinoma / colon cancer cell line (HCT116). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Reporter | GFP |
| Gene | SQSTM1 |
| Gene Species | Homo sapiens (human) |
| Host Cell | Human colorectal carcinoma / colon cancer cell line (HCT116) |
| Host Cell Species | Homo sapiens (human) cell line |
| Stability | This cell line is stable at least 10 passages. |
| Product Type | Human gene overexpression stable cell line |
| Applications | 1) investigation of gene function 2) screening and validation of antibodies |
| Quality Control | 1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Gene Name | Sqstm1 sequestosome 1 [ Mus musculus ] |
| Gene Symbol | Sqstm1 |
| Synonyms | Osi; p62; A170; STAP; OSF-6 |
| Gene Description | sequestosome 1 |
| Gene ID | 18412 |
| UniProt ID | Q64337 |
| mRNA Refseq | NM_011018.2 |
| Protein Refseq | NP_035148.1 |
| Chromosome Location | 11; 11 B1.2 |
| Function | K63-linked polyubiquitin binding; SH2 domain binding; identical protein binding; identical protein binding; metal ion binding; protein binding; protein homodimerization activity; protein kinase C binding; protein kinase binding; transcription cofactor activity; ubiquitin binding; zinc ion binding; |
| Pathway | Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem; Immune System, organism-specific biosystem; Interleukin-1 signaling, organism-specific biosystem; NF-kB is activated and signals survival, organism-specific biosystem; NRIF signals cell death from the nucleus, organism-specific biosystem; |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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We utilized this HCT 116 line for our proteostasis research. Compared to transient transfection, this stable line from Creative Biogene provides a much more uniform signal across the population. The recovery after thawing was nearly 100%, which allowed us to start our experiments immediately.
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