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Human IL6 Stable Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01388

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO01388
Description This cell line is engineered to stably express Homo sapiens (human) interleukin 6 (IL6) in Human immortalized embryonic kidney cell line (HEK293). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene IL6
Gene Species Homo sapiens (human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene IL6
Background This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]
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Exceptional Stability for IL-6 Bioactivity Assays

We utilize the Human IL6 stable cell line for our inflammation-related drug testing. The cells are very hardy and maintain constant IL-6 production, which is critical for our long-term experiments. Creative Biogene has provided us with a very reliable research tool.

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