Human IL5RA Stable Cell Line - TF-1

Name: Human IL5RA Stable Cell Line - TF-1
SKU: CSC-RO02485
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO02485

Host Cell : TF-1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO02485
Description	This cell line is engineered to stably overexpress Human IL5RA in TF-1 cells.
Product Type	Stable cell line constitutively expressing target gene(s)
Target Gene	IL5RA
Gene Species	Human
Host Cell	TF-1
Host Cell Species	Homo sapiens (Human)
Applications	1) Biological function research of the target gene(s), 2) Drug development of potential drug candidates targeting the target gene(s) or protein(s), 3) Investigation of disease mechanisms, 4) Antibody validation
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry, 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Suspension

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

IL5RA

Background

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]

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