FADS1 Easy KO Kit (Human)

Name: FADS1 Easy KO Kit (Human)
SKU: CC-493
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-493

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Gene Information

Cat. No.	CC-493
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Fads1
Gene Name	FADS1 fatty acid desaturase 1 [ Homo sapiens ]
Species	Human
EnsemblID	ENSMUSG00000010663
NCBIGeneID	76267
Uni ProtID	Q920L1
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	FADS1 fatty acid desaturase 1 [ Homo sapiens ]
Gene Symbol	FADS1
Synonyms	FADS1; fatty acid desaturase 1; LLCDL1; D5D; delta 5 desaturase; FADS6; FADSD5; TU12; delta-5 desaturase; delta desaturase; delta-5 fatty acid desaturase; delta fatty acid desaturase; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; FLJ38956; FLJ90273;
GeneID	3992
Uni ProtID	O60427
mRNA Refseq	BC007846
Chromosome Location	11q12-q13.1
Function	C-5 sterol desaturase activity; heme binding; oxidoreductase activity;
Pathway	Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem;
MIM	606148

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