Notch/RBP-Jk Luciferase Reporter Lentivirus

The Notch/RBP-Jκ luciferase reporter lentivirus is a ready-to-use, integratable reporter system designed to provide sensitive quantitative detection of classic Notch pathway activity. The vector is constructed based on a minimal promoter downstream of tandem RBP-Jκ response elements, ensuring low basal expression and a high dynamic range upon pathway activation, enabling precise detection of subtle signal changes. Lentiviral vector delivery allows for stable genomic integration and long-term reporter gene expression in a variety of cell types, including primary cells and difficult-to-transfect cell lines, overcoming the limitations of transient transfection and plasmid-based reporter systems. The system''s design ensures signal stability by minimizing off-target transcriptional noise, while luciferase as the reporter gene provides superior sensitivity and a broad linear range for reliable quantitative analysis.

This reporter system is ideal for real-time and long-term monitoring of Notch pathway dynamics in development, disease models, and drug discovery. It can quantify pathway activation induced by ligand stimulation or inhibition, thus aiding in the study of the mechanisms of action of antibodies, small molecules, or biologics that modulate Notch signaling. In oncology research, it can support functional analysis of Notch activity in the context of tumor cells and the microenvironment, contributing to the study of tumorigenesis, EMT programs, angiogenesis, and drug resistance mechanisms. In stem cell biology, the system can be used to track Notch-dependent self-renewal and lineage determination processes; in immunology, it can be used to report Notch-mediated T cell development and effector functions.