Mouse Il2ra Stable Cell Line - HEK293T

Name: Mouse Il2ra Stable Cell Line - HEK293T
SKU: CSC-RO0851
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0851

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0851
Description	This cell line is engineered to stably overexpress mouse IL2RA (also named as CD25).
Target Gene	Il2ra
Gene Species	Mus musculus (Mouse)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the origin of the Mouse Il2ra Stable Cell Line - HEK293T, and how was it established as a stable cell line?

A: The Mouse Il2ra Stable Cell Line - HEK293T is derived from HEK293T cells, a human embryonic kidney cell line that is commonly used in research. To establish this stable cell line, the Il2ra gene (Interleukin-2 receptor alpha) from mice was transfected into HEK293T cells, and stable clones were selected by introducing a selection marker, typically through antibiotic selection or other suitable methods.

What are the primary research applications of the Mouse Il2ra Stable Cell Line - HEK293T, and how does it contribute to the study of the Il2ra receptor?

A: The Mouse Il2ra Stable Cell Line - HEK293T is used primarily to study the Il2ra receptor and its interactions with ligands and signaling pathways. It serves as a valuable tool for investigating Il2ra-mediated immune responses, including T-cell activation, cytokine signaling, and immune regulation. Researchers often use this cell line to assess ligand-receptor binding affinity, downstream signaling cascades, and the impact of mutations or modifications on Il2ra function.

What specific genetic modifications or markers have been introduced into the Mouse Il2ra Stable Cell Line - HEK293T to ensure stable expression of the Il2ra gene?

A: To ensure stable expression of the Il2ra gene, researchers typically introduce genetic modifications such as a selection marker, such as a neomycin resistance gene (NeoR) or a green fluorescent protein (GFP) reporter gene. These markers allow for the selection and identification of cells that have successfully integrated and maintain the Il2ra gene. Stable expression is typically confirmed through molecular techniques like PCR, flow cytometry, or Western blotting.

What are some specific considerations or precautions researchers should be aware of when working with the Mouse Il2ra Stable Cell Line - HEK293T in their experiments?

A: When working with the Mouse Il2ra Stable Cell Line - HEK293T, researchers should consider the following precautions: Ensure proper culture conditions for HEK293T cells, including suitable media, supplements, and incubation conditions.

Can the Mouse Il2ra Stable Cell Line - HEK293T be used to screen for potential therapeutic agents or antibodies targeting Il2ra?

A: Yes, the Mouse Il2ra Stable Cell Line - HEK293T can be utilized in drug screening assays to identify potential therapeutic agents or antibodies that target the Il2ra receptor. Researchers can assess the binding affinity and functional effects of candidate compounds on Il2ra signaling. This approach may lead to the development of immunomodulatory drugs or biologics for conditions involving Il2ra dysregulation, such as autoimmune diseases.

Are there any emerging research areas or future directions in which the Mouse Il2ra Stable Cell Line - HEK293T is expected to play a significant role?

A: The Mouse Il2ra Stable Cell Line - HEK293T is expected to continue playing a significant role in various research areas, including: Investigating the role of Il2ra in autoimmune diseases and immunotherapy.

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