Cat.No. : CSC-RO0487 Host Cell: CHO-K1
| Cat. No. | CSC-RO0487 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Macaca mulatta SIGLEC15. |
| Gene | SIGLEC15 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey SIGLEC15 Stable Cell Line - CHO-K1 originates from monkey tissues and was likely obtained through cell culture techniques. These cells were originally derived from monkey kidneys or other tissues and then subjected to cell culture and stabilization processes to achieve the expression of the SIGLEC15 gene.
A: The expression of the SIGLEC15 gene in Monkey SIGLEC15 Stable Cell Line - CHO-K1 is achieved through gene transfection or gene editing techniques. Typically, researchers introduce the coding sequence of the SIGLEC15 gene into CHO-K1 cells and regulate its expression with an appropriate promoter. The phenotype of the SIGLEC15 gene involves the production of SIGLEC15 protein on the cell surface, which is a glycoprotein receptor involved in specific recognition and interaction with other molecules.
A: Monkey SIGLEC15 Stable Cell Line - CHO-K1 has multiple application areas in scientific research, including: Immunology Research: SIGLEC15 is a receptor on immune cells and is involved in regulating immune responses. This cell line can be used to study the interactions between immune cells and SIGLEC15 to gain insights into immune regulation mechanisms.
Drug Screening: Monkey SIGLEC15 Stable Cell Line - CHO-K1 can be employed to screen potential drug molecules that interact with SIGLEC15, aiming to discover new drug treatment approaches, especially for diseases related to the immune system.
A: When using Monkey SIGLEC15 Stable Cell Line - CHO-K1 in research, important ethical and regulatory considerations include: Animal Ethics: If the source of the cell line involves monkey tissues or animal experiments, research must comply with applicable animal ethics regulations and ethical review requirements.
Gene Editing Ethics: If gene editing techniques were used to construct this cell line, it is essential to ensure compliance with relevant gene editing ethics principles and regulations, including potential gene editing consequences and human applications.
A: When using Monkey SIGLEC15 Stable Cell Line - CHO-K1 in research, several biosafety considerations should be taken into account: Standard Operating Procedures: Ensure that laboratory members are trained and strictly adhere to standard operating procedures for cell culture, including wearing appropriate personal protective equipment.
Contamination Control: Take measures to prevent cross-contamination of cell cultures and regularly check the purity and cell morphology of cultures.
A: Yes, there may be relevant literature and research guidelines available for Monkey SIGLEC15 Stable Cell Line - CHO-K1. You can consult scientific literature databases, cell biology manuals, and research guidelines in the relevant field to obtain detailed information and usage recommendations for this cell line.
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